Our results revealed that MEOX2 and TWIST1 mRNA overexpression occurred in spite of the lack of significant differences in DNA methylation between the LNAT- and LT-derived samples, as previously described for TWIST1 in NSCLC [29], as well as MEOX2, which displayed few differences in DNA methylation between fetal lung, normal adult lung and the LT-derived samples (0%, 20% and 40%, respectively), but was overexpressed in the LT-derived samples [30]. The gene discussed is TWIST1; the disease is non-small cell lung carcinoma.