Taken together, these results indicate that core EMT-TFs including Zeb1, Zeb2, Snai1, Snai2 and Twist1 are potentially the specific substrates of the atypical E3 ligase complex SPFFbxo45, which mediates their degradation thereby controlling the occurrence of tumor cell EMT processes and even tumor metastasis. The gene discussed is TWIST1; the disease is neoplasm.