To determine if the differences in the pathogenicity of the LPHVs could be related to possible differences in their growth or replication kinetics, we compared their replication rates in different cell lines including IFN-deficient Vero E6 cells, IFN competent HuH-7 cells and IFN competent Tb1Lu cells using qRT–PCR to measure the production of genomic RNA in infected culture supernatants at different times after infection. The gene discussed is IFNA1; the disease is infection.