To investigate TLR3 expression in human PCa and its association with IRF-3 expression, sections from 30 human prostate samples, harbouring cancers of different grade (10 tumours G1 = Gleason score ≤6; 10 tumours G2 = Gleason score 7; 10 tumours G3 = Gleason score ≥8) were immunostained with anti-TLR3 and anti-IRF-3 antibodies. The gene discussed is TLR3; the disease is cancer.