TCF7 and metastatic prostate carcinoma: Further confirming the correlation between Ras pathway dysregulation and WNT signaling in these cells, western blots for GTP-Ras and phospho-p38MAPK showed relatively increased Ras signaling and TCF7 level in PC3 and RasB1 cells (Figure 2B), suggesting a synergistic action of Ras and the WNT pathway in metastatic prostate cancer cells.