Univariate analysis identified the following significant prognostic factors for poor DFS: serum AFP level ≥400 ng/ml, serum GGT level ≥50 U/L, incomplete tumor capsule, macrovascular invasion, tumor size ≥10 cm, tumor number ≥3, poor degree of tumor cell differentiation , and operation time >180 min (Table S3). This evidence concerns the gene AFP and neoplasm.