A representative dot plot analysis for CD4+ cells from an OVC sample is shown in Figure 1A. Using the gating scheme in Figure 1A we found that the mean percentage of CD4+ CD25+ FoxP3+ Treg within primary and omental mets in ovarian cancer patients were significantly increased compared to PBL (Figure 1C); however, in CRLM patients there was no significant difference in the mean percentage of Treg in TIL compared to blood (8.1% and 10.2% respectively, Figure 1B). This evidence concerns the gene FOXP3 and ovarian cancer.