To ascertain the influence of the genetic modification of T-cells with N1/28z on T-cell cytotoxic potential, we first performed cell-mediated cytotoxicity assays; following the co-culture of transduced CD8+ T-cells with CFSE-labeled tumor cells, we observed a statistically significant anti-tumor cytotoxic activity mediated by N1/28z-transduced lymphocytes as exemplified by the PI-positive population (Fig. 5A - 58 % for N1/28z vs. 11 % for NGFR using the HeLa target cell-line; p=0.05). The gene discussed is CD8A; the disease is neoplasm.