To further address the question why NSC745885 selectively down-regulated EZH2 in bladder cancer cells than in normal urothelial cells, the phosphorylation status at serine 21 and threonine 487 of EZH2 was examined in MBT2, T24, and SV-HUC-1 cells. The gene discussed is EZH2; the disease is urinary bladder carcinoma.