In our current study, we investigated KIF18A expression in HCC patients and found that KIF18A expression at both mRNA and protein level was significantly up-regulated in liver cancer tissues compared with that in ANLT, we also found a positive correlation between KIF18A expression and clinical characteristics, including AFP ≥200 ng/ml, tumor size >5 cm, TNM stage III/IV and PVTT appearance, which are closely related to a bad outcome of HCC. Here, KIF18A is linked to hepatocellular carcinoma.