CLCN3 and neoplasm: An increase in apoptosis, detected by using cleaved caspase 3 (ClC3) immunostaining, was observed in BKM120-treated tumors compared with that of controls (twofold increase), but the dual treatment resulted in a marked greater effect, inducing a fivefold increase in apoptotic tumor cells over those exposed to the single drug treatment and a tenfold increase compared with that of vehicle-treated cells (Fig. 6B,C).