Castro and colleagues also showed that rainbow trout are able to induce a typical antibody response, with evidence of specific clonal expansions, in their spleen following infection with VHSV (Viral Haemorrhagic Septicaemia Virus), a natural pathogen of this species; IgM showed the greatest repertoire shift post-infection with IgT being involved in the splenic response to a lesser degree and the IgD repertoire being modified only to a very low level [84]. This evidence concerns the gene CD40LG and infection.