In contrast, co-incubation with either AC133+ CD133-OE U251 cells (Figure 2E, left) or NCH421 GBM-SCs (not shown) caused detachment of the target cells and rosette formation, indicating clumping of antigen-expressing tumor cells with AC133-specific CAR T cells and proliferation of the latter. This evidence concerns the gene PROM1 and glioblastoma.