After incubation with NCH421k GBM-SCs and subsequent upregulation of CD57, our AC133-specific CAR T cells still proliferated when further cultured on anti-CD3 antibody-coated plates or with AC133+ NCH421k GBM-SCs for several days and they downregulated neither CD27 nor CD28 (data not shown), suggesting that the strong CD57 upregulation on the CAR T cells upon incubation with GBM-SCs did not reflect terminal replicative senescence, nor was it identical to the state of incomplete T cell differentiation recently described by Wu et al. [33]. This evidence concerns the gene CD28 and glioblastoma.