To target the TAZ gene, we integrated Cas9 under control of Tet-On transactivator into the genome of PGP1 (ref. 15) hiPSCs via the PiggyBac transposon and transfected the cells with a gRNA targeting a region (chr.X: 153,647,923–153,647,944) close to the mutation found in Barth Syndrome patients16. This evidence concerns the gene TAFAZZIN and Barth syndrome.