To the opposite, the expression level of HER2 in SKOV3-T was dramatically lower than SKOV3by flow cytometry method (Figure 2A).To further examine the role of IGF-1R in ovarian cancer cells, IGF-1R-pCMV6 plasmid, a eukaryotic expression vector subcloned with full IGF-1R exon sequence, was transfected into SKOV3 using lipofectamine 2000, generating a pool of IGF-1R-positive SKOV3 cells (Figure 2B). The gene discussed is ERBB2; the disease is ovarian cancer.