Furthermore, we also assayed the level of IGF2 in the brain tissues of six NTD-affected cases, of whom the clinical phenotypes were two occipital encephalomeningoceles (20-gestation week, female; 22-gestation week, male); one anencephaly and holorachischisis (20-gestation week, female); one anencephaly and parietal encephalomeningocele (22-gestation week, female); one occipital encephalomeningocele and cervical, thoracic spina bifida (37-gestation week, female); and one parietal encephalomeningocele (24-gestation week, male). This evidence concerns the gene IGF2 and neural tube defect.