The present study aimed to investigate the diagnostic value of ICC to detect M. tuberculosis specific antigen, MPT64, for the diagnosis of the most common presentations of EPTB, TBLN and TBP in pleural fluid and lymph node aspirate samples from Tikur Anbessa Specialized Hospital and United Vision Medical Services, Addis Ababa, Ethiopia. The gene discussed is TBP; the disease is intrahepatic cholangiocarcinoma.