EPCAM and neoplasm: The specificity and sensitivity of EpCAM as a marker of human tumor cells, was tested by triple and quadruple staining combining anti-EpCAM, anti-Tra-1-85 (pan anti-human antibody, filled blue), anti-H2Kd (mouse MHC class I antibody, red line) and Hoecst-3342 (DNA-content, grey contour), with subsequent flow cytometry analysis (Figure 1A and B, right panel).