While we did not measure changes in insulin signaling in this cohort of animals, others have shown consistently that rapamycin affects the insulin pathway in much the same manner in multiple strains of mice including C57BL6, namely creating glucose intolerance, but causing insulin sensitivity (Lamming et al., 2013; Orr et al., 2014; Yu et al., 2014). The gene discussed is INS; the disease is Glucose intolerance.