8 of these cases displayed a nuclear localization, corresponding to 6 SCLC, 1 LCNEC and 1 AC (Additional file 3).None of the transcriptional repressors (Snail1, Snail2, Twist and Foxc2) were expressed in the healthy respiratory epithelium (Figure 1D-1G). The gene discussed is TWIST1; the disease is large cell neuroendocrine carcinoma.