NFKBIA and colorectal cancer: HCT-116 p53+/+ cells are often used as a colorectal cancer model because they overexpress β-catenin.26 In contrast to HT-29 cells, HCT-116 p53+/+ cells show a decreased nuclear translocation of p65 in the presence of 2.5–25 μM BITC (Figure 4a), accompanied by a decreased phosphorylation of IκB-α at Ser32/36 (Supplementary Figure 2).