To determine the influence of parasite dose on the early expansion and activation of different T cell subsets following L. major infection, we co-cultured CFSE-labeled dLN cells from mice infected with low and high dose L. major (one week post-infection) with L. major-infected BMDCs and assessed T cell proliferation and cytokine (IFN-γ and TNF) production by flow cytometry. Here, TNF is linked to infection.