Among them, LAKs, which are a mixture of lymphokine-activated CD3+ T lymphocytes and CD3−CD56+CD16+ NK cells, were cultured with recombinant interleukin-2 (rIL-2) for 3 days, and CTLs were isolated from a patient's own tissues, including peripheral blood mononuclear cells (PBMCs), TILs, draining lymph nodes, or PBMCs after vaccination with irradiated autologous tumor cells (ATCs) [7], [8]. The gene discussed is IL2; the disease is neoplasm.