Interestingly, at around the same dose of A23187 that induced B220 expression, 50% of HPB-ALL, Jurkat, BW5147 and EL-4 cells died (Annexin V and PI staining, Figure 5, see Cell viability) or shifted from region R2 to R1 on the FSC vs. SSC dot plots (Figure 5, see Cell morphology). The gene discussed is ANXA5; the disease is acute lymphoblastic leukemia.