In this study, we detected the expression levels of MDM4S and MDM4FL in CK-AML patients with wild-type TP53. We also measured cell proliferation, cell cycle, proteins related to p53 pathway and spindle checkpoint expression levels, and analyzed karyotypes in MDM4-overexpressing tumor cell line with wild-type TP53. Here, TP53 is linked to acute myeloid leukemia.