TP53 mutation and deletions were detected by genome PCR sequencing and interphase FISH in 24 CK-AML cases, and 15 CK-AML cases were wild-type TP53. In order to rule out other genes mutation associated with poor prognosis of CK-AML, we detected RUNX1 mutations in 15 CK-AML patients and FLT3-ITD mutation in 131 de novo AML cases (15 patients with wild-type TP53 and 116 NK-AML patients). This evidence concerns the gene RUNX1 and acute myeloid leukemia.