GAD65121–140- and GAD65250–266-specific CD4+ T cell responses were detected more often in T1D subjects than controls (GAD65121–140: 6/10 T1D and 0/9 controls (p = 0.0108); GAD65250–266: 9/17 T1D and 1/11 controls (Table 2, p = 0.0407); p value evaluated by two-tailed Fisher's exact tests), demonstrating a clear association of DQ8-restricted GAD65 T cell responses with disease. Here, GAD2 is linked to type 1 diabetes mellitus.