Fig. 5A shows the possible regulation network between miRNAs and mRNAs. Then, we combined the in silico analysis of miR-429 targets and global transcriptional profile (Fig. 5B). Among all these results, CRKL was the target gene on both sides, indicating this gene was probably a fundamental node in controlling bone metastasis of breast cancer. Moreover, immunoblot assay showed that miR-429 reduced ZEB1 and CRKL significantly, which is a classical EMT inducer in breast cancer (Fig. 5C). This evidence concerns the gene CRKL and breast cancer.