On the basis of a cut-off value of +2.5 fold relative to controls, 9 different breast cancer-related genes, other than BRCA1, were highly methylated in the breast cancer cases compared to controls (Figure 2), HIC1 (p = 0.093), CDH13 (p = 0.014), CDH1 (p = 0.011), CDKN2A (p = 0.167), MGMT (p = 0.067), SLIT2 (p = 0.013), CCNA1 (p = 0.075), TNFRSF10C (p = 0.029) and PYCARD (p = 0.269). This evidence concerns the gene PYCARD and breast cancer.