In the current study, we sought to further characterize the properties of TnTs in mesothelioma, including differences in formation of TnTs between malignant mesothelioma cells and non-malignant mesothelial cells; quantitative differences in TnT length in relation to cell proliferation; properties of TnT formation in clinically relevant models, such as between non-adherent cells, mimicking the scenario of mesothelioma cells floating in peritoneal or thoracic effusions as a hallmark of malignant progression; and structural components of TnTs in mesothelioma cells. Here, TNNT1 is linked to cancer.