Using the human hepatocellular carcinoma cell line HepG2, we also showed that Nck1 depletion by siRNA promotes insulin signaling, as represented by increased levels of pY IRS-1, Akt phosphorylation on Ser473, GSK3β phosphorylation on Ser9 and glycogen synthesis in response to insulin [24]. Here, INS is linked to hepatocellular carcinoma.