APC and malignant colon neoplasm: We used two colon cancer cell lines that are representative of two different Wnt genetic contexts: RKO cells, which express a normal APC and display normal canonical Wnt signaling that depends on the ligand for activation, and SW480 cells, which only express mutant truncated APC and are representative of colon cancer cells that display constitutively active canonical Wnt signaling [16].