CLDN1 expression was significantly higher in the LN− group, 55.3% (42/76) compared to 36.1% (35/97) in the LN+ group, suggesting that the absence of CLDN1 expression is related to lymph node metastasis (P = 0.014) (Table 2). Here, CLDN1 is linked to metastatic malignant neoplasm in the lymph nodes.