Moreover, our extension analysis of 96 patients using targeted sequencing of 1,209 genes, revealed a number of truncation mutations in TP53, RB1, PTEN, PTPRD, ARID2, ASXL3, and other genes co-occuring with BRAF and NRAS mutations, suggesting their cooperating roles during the progression of melanoma. The gene discussed is ASXL3; the disease is melanoma.