The aim of the present study was to extend our stroke in vitro models of the BBB with astrocytes by co-cultivation of mouse BBB cell line cerebEND with rat cell line C6 and to investigate the influence of astrocytes under OGD-conditions on several BBB relevant parameters such as functionality of the physical as well as transport barrier, associated tight junction molecule and Abc-transporter expression, expression and functionality of MMPs, t-PA and of their endogenous inhibitors, and last but not least whether morphological changes were detectable via atomic force microscopy. This evidence concerns the gene PLAT and stroke disorder.