With an aim to contribute to the elucidation of the role of CFTR gene in the impaired spermatogenesis and male infertility, we designed a screening assay for 11 most common CFTR gene mutations and IVS8polyT variant, based on primer extension with fluorescent dideoxynucleotides and subsequent detection on capillary electrophoresis. The gene discussed is CFTR; the disease is male infertility.