To test the efficacy of the NfκB nuclear traslocation, we also compared our data with a TNFα pro-inflammatory treatment and with MM EVs pre-coated with the CD63 antibody: as expected we obtained a significant NfκB nuclear traslocation compared to control cell (starvation) both in immunofluorescence and WB analysis (Figures 6A–C). Here, TNF is linked to Miyoshi myopathy.