Statistical analysis revealed that the number of IGF-1-expressing cells is significantly higher in L-SIL, H-SIL and ICC versus the control (Kruskal-Wallis test, P=0.032; Dunn’s post-test, P=0.034, P=0.013 and P=0.001 for L-SIL, H-SIL and ICC, respectively). The gene discussed is IGF1; the disease is intrahepatic cholangiocarcinoma.