GPT and cholestasis: As shown in Table 1, increased enzyme values (i.e., ALP, ALT, and gammaGT) indicating cholestasis and stage determination did not reveal a statistical difference between the two groups of patients, though the majority of AMA-positive PBC patients were in stage 3 (characterized by fibrous scaring bridging portal tracts with occasional foci of bile duct loss), whereas AMA-negative were in stage 2 (showing portal enlargement with bile ductular reaction and inflammatory cell infiltration).