Of interest, when we stratified all PBC patients as low (i.e. 0.1–10 ng/ml), and high sCTLA-4 concentration (i.e. >10.1 ng/ml), we could observe a positive correlation between sCTLA-4 and the PBC screen test, IIF-AMA results, MIT3, and gp210 presence (Table 2). Here, NUP210 is linked to primary biliary cholangitis.