To further investigate the connection between Msi expression and EMT in breastcancer, we examined Msi expression in a panel of breast cancer-derived cell lines.Consistent with the RNA-Seq data from primary tumors, HER2+ epithelial celllines expressed higher levels of Msi1 and Msi2compared with HER2– lines (Figure 2D,lane 6 and 7). The gene discussed is MSI1; the disease is breast cancer.