Consistent with the IFI16 knockdown experiments (Figure 1), though expression of each gene increased over time regardless of the presence of overexpressed IFI16 (Figure 2C), IFI16 overexpression significantly inhibited expression of HSV-1 genes from all gene classes over the course of 8 h of infection: compared to GFP-transduced cells, in IFI16-transduced cells at 8 h p.i., ICP0 was inhibited by >50%, ICP4 was inhibited by 76%, ICP8 was inhibited by 50%, TK was inhibited by 87%, gB was reduced by 74%, and Us11 was inhibited by 77% (Figure 2D). The gene discussed is IFI16; the disease is infection.