Our results are in accordance with the results found with breast cancer cells9 and B-Raf-mutated melanoma cells.40 We also found that bortezomib treatment resulted in p62 accumulation in hepatocellular carcinoma HepG2 cells and endometrial cancer Ishikawa cells, indicating that autophagic flux was blocked by bortezomib. This evidence concerns the gene BRAF and hepatocellular carcinoma.