Treatment of human colon cancer cells with curcumin induced the conversion of LC3-I to LC3-II and degradation of SQSTM1 and autophagosomes.104 The autophagic changes induced by curcumin were almost completely blocked in the presence of the antioxidant NAC, indicating that treatment led to ROS production, autophagosome formation, and autolysosomal degradation. This evidence concerns the gene SQSTM1 and malignant colon neoplasm.