After confirming functionality of this approach with training RNA-seq datasets from neuroblastoma CAD and fibrosarcoma L929 cells (Fig. S1 and Tables S1 and S2) we repeated the analysis for our RNA-seq datasets obtained for CAD cells transfected with control siRNA, Ptbp1-specific siRNAs or a mixture of siRNAs against Ptbp1 and its brain-enriched paralog Ptbp2 with a largely overlapping AS regulation preferences [26], [36], [37] (siControl, siPtbp1 and siPtbp1/2, respectively; [29]; NCBI Gene Expression Omnibus accession number GSE37933). This evidence concerns the gene PTBP2 and neuroblastoma.