Quantification of robo2-GFP expressing cells in the CySC lineage [31] revealed a significant increase in the number of Robo2-GFP-positive CySCs and early cyst cells following Upd overexpression (43.9 vs. 31.4 cells per testis, p<.0001, n>17), as well as a 38% increase in normalized GFP pixel intensity (p<.01, Figure 5E, 5F). Here, ROBO2 is linked to cyst.