To dissect the impact of p12 and/or p8 on CTL responses in the context of the whole virus, we generated immortalized infected CD4+ T-cells lines from an HLA.A2 healthy donor that allowed the use of the human CTL clone from a HAM/TSP patient that recognize the HLA.A2 restricted Tax peptide [11]–[19], [29]. This evidence concerns the gene CD4 and tropical spastic paraparesis.