The pEEV was used to deliver via electroporation DNA encoding for GmCSF and b7.1 (pEEVGmCSF-b7.1), which we have demonstrated protects from primary and secondary tumour growth in both the colon adenocarcinoma and the melanoma cancer models by generating a robust proinflammatory immune cell recruitment and cytokine environment in both systemic and tumour compartments. Here, CSF2 is linked to colon adenocarcinoma.