CD80 and cancer: Previously, we have shown that delivery of our EEV plasmid (pEEV) via electroporation is capable of achieving reliable and superior expression in a variety of murine and porcine tissue types when compared with a control plasmid.26 We therefore decided to engineer pEEV to contain the immune therapeutic genes: GmCSF and b7.1, and test their efficacy in two aggressive cancer models.