2F). In vitroanchorage independent tumorigenic activity of TE9 ESCC cells was also significantly decreased in shZNF282-treated group as shown in soft agar colony formation assay (Figure. 2G). These results suggest that ZNF282 promotes migration / invasion and facilitates anchorage independent tumor growth of ESCC. This is well consistent with our clinical observation previously mentioned that ZNF282 overexpression was positively correlated with advanced T stage inESCC because T stage indicates the extent and depth of direct tumor invasion (Table 2). The gene discussed is ZNF282; the disease is esophageal squamous cell carcinoma.