In the present study, phosphorylation of AMPKα, Bad, PRAS40, SAPK/JNK and GSK3β, as well as cleavage of PARP was elevated in eIF3D knockdown group, adding the evidence that depletion of eIF3D could suppress colon cancer cell growth via the induction of cell-cycle arrest and apoptosis. This evidence concerns the gene AKT1S1 and malignant colon neoplasm.