In the present study, phosphorylation of AMPKα, Bad, PRAS40, SAPK/JNK and GSK3β, as well as cleavage of PARP was elevated in eIF3D knockdown group, adding the evidence that depletion of eIF3D could suppress colon cancer cell growth via the induction of cell-cycle arrest and apoptosis. Here, MAPK8 is linked to malignant colon neoplasm.