To test the role of TcpF in NF-κB activation during enterococcal infection, we first compared the nuclear translocation of NF-κB-p65 (a subunit of the NF-κB transcription complex) during infection of mouse RAW264.7 macrophages with strains E99, SPB03 or SPB04, by Western blot. This evidence concerns the gene NFKB1 and infection.