Our transcriptome analysis revealed that over-expression of miR-27 in human embryonal carcinoma cells leads to down-regulation of pluripotency-associated genes, such as GDF3, LIN28, TRIM71, DNMT3A, DNMT3B and USP46 and an activated expression of developmental genes such as SMAD6, BMP2, FST and HAND1 (Figure 5C). This evidence concerns the gene HAND1 and embryonal carcinoma.